45 ticks (n = 9) significantly differed for B. burgdorferi (s.l.), Rickettsia spp., and "Ca. N. mikurensis", but were not related to the habitat type. Three hundred fifty eight out of 1078 I. ricinus ticks (33.2%) tested positive for at least one pathogen. Thereof, about 20% (71/358) were carrying two or three different potentially disease-causing agents. Using next generation sequencing, we could detect true pathogens, tick symbionts and organisms of environmental or human origin in ten selected samples. Conclusions Our data document the presence of pathogens in the (sub-) urban I. ricinus tick population in Switzerland, with carrier rates as high as those in rural regions. Carriage of multiple pathogens was repeatedly observed, demonstrating the risk of acquiring multiple infections as a consequence of a tick bite."/>
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Table 4 Tick collection success with respect to collection dates for sites with multiple collection attempts

From: Prevalence of tick-borne pathogens in questing Ixodes ricinus ticks in urban and suburban areas of Switzerland

Collection sites Number of I. ricinus ticks collected
City Name June 2015 July 2015 September 2015 November 2015 April 2016 May 2016
Zürich Waldrand Waid, Chäferberg 8 0 6 0 13 20
Zürich Waidberg Wald 9 21 19 3 42 49