Table 1 Target genes and primers used in this study [26, 27]
From: Molecular and serological detection of bovine babesiosis in Indonesia
Species | Target gene | Method | Primer name | Oligonucleotide sequence (5′–3′) | Product size (bp) |
|---|---|---|---|---|---|
B. bovis | SBP-4 | PCR | bov-SBP-4-F | AGTTGTTGGAGGAGGCTAAT | 907 |
| Â | Â | bov-SBP-4-R | TCCTTCTCGGCGTCCTTTTC | ||
| Â | Â | nPCR | bov-SBP-4-nF | GAAATCCCTGTTCCAGAG | 503 |
| Â | Â | bov-SBP-4-nR | TCGTTGATAACACTGCAA | ||
B. bigemina | RAP-1a | PCR | big-RAP-1a-F | GAGTCTGCCAAATCCTTAC | 879 |
| Â | Â | big-RAP-1a-R | TCCTCTACAGCTGCTTCG | ||
| Â | Â | nPCR | big-RAP-1a-nF | AGCTTGCTTTCACAACTCGCC | 412 |
| Â | Â | big-RAP-1a-nR | TTGGTGCTTTGACCGACGACAT | ||
B. bovis | ITS region | PCR | bov-ITS-F | CGTCCCTGCCCTTTGTA | 815 |
| Â | Â | Â | bov-ITS-R | TATTTTCTTTTCTGCCGCTT | Â |
| Â | Â | nPCR | bov-ITS-nF | CACCACCAGTGGAAGCAC | 545 |
| Â | Â | Â | bov-ITS-nR | TTGTGCCCCATGGACACT | Â |
B. bigemina | ITS region | PCR | big-ITS-F | CGTCCCTGCCCTTTGTA | 1041 |
| Â | Â | Â | big-ITS-R | TATTTTCTTTTCTGCCGCTT | Â |
| Â | Â | nPCR | big-ITS-nF | AGTGGTCGGGACTCGTC | 495 |
| Â | Â | Â | big-ITS-nR | AGTACCGCGTGCGAGCAG | Â |