Production and characterization of monoclonal antibodies against Encephalitozoon intestinalis and Encephalitozoon sp. spores and their developmental stages

Table 1 Reactivity and characterization of MAbs to E. intestinalis

MAb	Immunization protocol	Isotype	ELISA (OD)	IFAT^a	WB (KDa)^b		EM	Specificity of the MAbs (use dilution by IFAT)
MAb	Immunization protocol	Isotype	ELISA (OD)	IFAT^a	E. intestinalis	E. cuniculi / E. hellem	EM	Genus	E. intestinalis
4C4	1	IgG_2a	+ (1.482)	+	201–36.4 (45)^c	205–36.8 (63 & 59.2)^c	EXP/ENP	1:25–1:50	1:400^d
2C2	3	IgG_2a	+ (0.940)	+	201–36.4 (45)^c	205–36.8 (63 & 59.2)^c	EXP/ENP	Not diluted	≥ 1:50^d
2H2	3	IgG_2a	+ (0.859)	+	201–36.4 (45)^c	205–36.8 (63 & 59.2)^c	EXP/ENP	Not diluted	≥ 1:50^d
2E5	3	IgG_2a	+ (0.732)	+	48.3–36.4 (45)^c	205–36.8 (63 & 59.2)^c	EXP/ENP	Not diluted (negative for E. hellem)	≥ 1:25^d
3A5	2	IgG₃	+ (1.130)	–	16	–	CTPC	–	–
3C9	2	IgG₃	+ (1.523)	–	40 & 16	–	CTPC	–	–
1D7	2	IgM	+ (0.756)	–	40 & 16	–	CTPC	–	–

^aMature spores
^bSoluble antigen
^cWell-defined band
^dWithout cross-reactions
Abbreviations: MAb monoclonal antibody, + positive reactivity, − no reactivity, OD optical density, EM electron microscopy, EXP exospore, ENP endospore, CTPC cytoplasmic contents

ISSN: 1756-3305