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Fig. 2 | Parasites & Vectors

Fig. 2

From: Hap2, a novel gene in Babesia bigemina is expressed in tick stages, and specific antibodies block zygote formation

Fig. 2

Analysis of HAP2 expression in sexual and asexual stages of Babesia bigemina by indirect immunofluorescence. B. bigemina sexual stages at 22 and 36 h post-induction, and asexual stages at 0 h were evaluated with antibodies against HAP2. a-c Sexual stages 22 h post-induction. e-g Sexual stages at 36 h post-induction. d, h Infected erythrocytes. a, e Giemsa stained sexual stages at 22 and 36 h post-induction, respectively. Sexual stages at 22 h (b), or at 36 h post-induction (f), as well as asexual, intraerythrocytic merozoites (d), were incubated with anti-HAP2 antiserum. Sexual stages at 22 h (c), or at 36 h post-induction (g), as well as asexual, intraerythrocytic merozoites (h) were incubated with pre-immune serum (1000×)

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