Fig. 2From: Hap2, a novel gene in Babesia bigemina is expressed in tick stages, and specific antibodies block zygote formationAnalysis of HAP2 expression in sexual and asexual stages of Babesia bigemina by indirect immunofluorescence. B. bigemina sexual stages at 22 and 36 h post-induction, and asexual stages at 0 h were evaluated with antibodies against HAP2. a-c Sexual stages 22 h post-induction. e-g Sexual stages at 36 h post-induction. d, h Infected erythrocytes. a, e Giemsa stained sexual stages at 22 and 36 h post-induction, respectively. Sexual stages at 22 h (b), or at 36 h post-induction (f), as well as asexual, intraerythrocytic merozoites (d), were incubated with anti-HAP2 antiserum. Sexual stages at 22 h (c), or at 36 h post-induction (g), as well as asexual, intraerythrocytic merozoites (h) were incubated with pre-immune serum (1000×)Back to article page