Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)

Table 2 Effects of the height of the larval rearing trays in units on pupal production and larval survival

Unit	Layers of unit	Water temp (°C)	34 h (Mean ± SE)
Unit	Layers of unit	Water temp (°C)	PP	M%	Larval survival (%)^a
Wol-unit	Top	26.7 ± 0.2	3399 ± 101 a	76.9 ± 1.6 a	99.3 ± 1.9 a
	Middle	26.4 ± 0.2	3247 ± 137 a	77.2 ± 2.0 a	98.5 ± 1.3 a
	Bottom	26.2 ± 0.1	2873 ± 219 a	79.2 ± 2.4 a	98.3 ± 0.8 a
IAEA-unit	Top	28.4 ± 0.2	10,390 ± 704 A	73.5 ± 0.7 A	98.3 ± 0.4 A
	Middle	27.4 ± 0.3	9407 ± 552 A	75.0 ± 1.3 A	96.9 ± 3.5 A
	Bottom	27.7 ± 0.3	9100 ± 847 A	74.5 ± 1.7 A	96.6 ± 0.5 A

^aLarval survival was calculated as: (Total number of pupae collected + Residual number of larvae after sex separation) / (Estimated number of larvae per tray)
Note: Within each column, values followed by different lowercase or capital letters were statistically different using ANOVA analysis and Tukey post-hoc test (P < 0.05)
Abbreviations: PP pupae production, M% percentage of male pupae in PP

ISSN: 1756-3305