Establishment of a medium-scale mosquito facility: optimization of the larval mass-rearing unit for Aedes albopictus (Diptera: Culicidae)

Table 3 Pupae production and sex separation efficiency by using the entire larval rearing units

Parameter	Wol-unit	IAEA-unit
Number of trays per rack	40	50
Estimated number of male pupae per tray^a	2459	7144
Estimated male pupae production per unit (10⁵)^b	0.98	3.57
Male pupae production per unit (10⁵)	0.89 ± 0.02	3.16 ± 0.11
Female pupal contamination rate (%)	0.5 ± 0.1 a	0.9 ± 0.2 a
Male pupae production efficiency (%)^c	90.9 ± 2.4 a	88.5 ± 3.0 a

^aEstimated number of male pupae per tray for Wol-tray and IAEA-tray was obtained from the top, middle and bottom section of larval rearing unit
^bEstimated male pupae production per unit was calculated as: (Number of trays per unit × Estimated number of male pupae per tray)
^cMale pupae production efficiency was calculated as: (Male pupae production per unit / Estimated male pupae production)
Note: Within each row, values followed by same lowercase letters were not statistically different using Mann-Whitney U-test analysis (P > 0.05). All the data in the table were presented as Mean or (Mean ± SE)

ISSN: 1756-3305