Fig. 1
Phylogenetic tree forĀ Opisthorchis sp. BD2013 (indicated by diamond symbol) and other opisthorchiids and representative trematodes from 4 families, theĀ Opisthorchiidae, Heterophyidae, Fasciolidae and Schistosomatidae (the latter used as an outgroup), based on concatenated nucleotide sequences of complete cytochrome b (cob), nicotinamide dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) genes. Phylogenetic reconstruction was performed using maximum likelihood analysis with the general time-reversible model with a gamma distributed rate heterogeneity and a proportion of invariant sites (GTR + Ī+ I) in the MEGA6.06 software package. Support for each node was evaluated using 1000 bootstrap resamplings [21]. The scale-bar indicates the number of substitutions per site. Accession numbers (where available) are given at the end of each sequence name. Isolates/geographical localities are given in parentheses (if available). Country abbreviation codes (2-letter) given prior to the accession numbers: AU, Australia; CN, China; CZ, Czech Republic; KR, Korea; LA, Lao PDR; RU, Russia; TH, Thailand; VN, Vietnam