Fig. 1
From: Successful long-term maintenance of Mansonella perstans in an in vitro culture system
In vitro long-term maintenance of M. perstans infective larvae co-cultured with monkey kidney epithelia cells (LLC-MK2). M. perstans infective larvae (L3; n = 15) were co-cultured with monkey kidney epithelia cells (LLC-MK2) in DMEM medium supplemented with 10% FBS at 37 °C and 5% CO2 for 77 days. Cultures were monitored microscopically to determine survival rates (%) of M. perstans worms