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Fig. 2 | Parasites & Vectors

Fig. 2

From: Successful long-term maintenance of Mansonella perstans in an in vitro culture system

Fig. 2

Mansonella perstans infective larvae moult and developed into viable and motile worms in vitro. M. perstans infective larvae (n = 249) were co-cultured with monkey kidney epithelia cells (LLC-MK2) in DMEM medium supplemented with 10% FBS at 37 °C and 5% CO2 for 50 days. The cultures were monitored on a daily basis via microscopy to determine (a) worm motility (%) according to a 4 point scale [0, no movement; 1, intermittent shaking of head and tail; 2, sluggish (shaking of the whole worm on a spot); and 3, vigorous movement] and (b) moulting rates (%) of the developing M. perstans worms

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