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Fig. 2 | Parasites & Vectors

Fig. 2

From: SjCRT, a recombinant Schistosoma japonicum calreticulin, induces maturation of dendritic cells and a Th1-polarized immune response in mice

Fig. 2

SjCRT expression from RA and normal schistosomula at different stages. Cercariae were exposed or not exposed to a UV source (254 nm) at an intensity of 400 μW/cm2 for 1 min and the tails were detached before culture for 4, 7, 10 or 14 days. Schistosomula were digested into single cells and the cytomembrane was ruptured with 0.2% Triton-×100 on ice for 10 min. Then the cells were collected to determine the expression of SjCRT by flow cytometry. Data illustrating representative experiments and derived from triplicate experiments are shown as a graph (a1-a4) and mean fluorescence intensities (b1-b4) showing the means ± SD from 4 samples, representative of two separate experiments. Statistically significant differences compared with the same stage of normal groups are shown as *P < 0.05 or **P < 0.01 (b1: t (3) = 4.571, P = 0.014; b2: t (3) = 5.537, P = 0.0311; b3: t (3) = 9.415, P = 0.0025; b4: t (3) = 6.629, P = 0.0070)

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