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Fig. 3 | Parasites & Vectors

Fig. 3

From: SjCRT, a recombinant Schistosoma japonicum calreticulin, induces maturation of dendritic cells and a Th1-polarized immune response in mice

Fig. 3

FITC-SjCRT binds to DCs and can be engulfed by DCs through CD91. a FITC-SjCRT binds to the surface of DCs at 4 °C. b FITC-SjCRT is endocytosed by DCs at room temperature. c Control of the same visual field. DCs fixed in paraformaldehyde were incubated with 20 μg/ml unlabeled SjCRT, 100 μg/ml unlabeled SjCRT and α2-M at 4 °C for 30 min, and 2 μl FITC-SjCRT (2 μg/ml the final concentration) was then added at 4 °C for 20 min. Then, cells were washed twice with precooled PBS, resuspended in 300 μl precooled PBS and analyzed by flow cytometry. Data illustrating representative experiments derived from quadruplicate experiments (d) and mean fluorescence intensities (e) are shown. Statistically significant differences compared with the control (FITC-SjCRT group) group are shown as *P < 0.05 or **P < 0.01 (100 μg/ml SjCRT: t (3) = 15.66, P = 0.0041; 20 μg/ml SjCRT: t (3) = 4.038, P = 0.0482; α2-M t (3) = 15.93, P = 0.0039)

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