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Fig. 4 | Parasites & Vectors

Fig. 4

From: SjCRT, a recombinant Schistosoma japonicum calreticulin, induces maturation of dendritic cells and a Th1-polarized immune response in mice

Fig. 4

Phenotypic and functional maturation of DCs induced by rSjCRT protein. DCs were treated with rSjCRT, LPS or PBS for 48 h, and cells were then collected for FACS analysis of CD40 and CD86 expression. CD40: LPS t (3) = 5.506, P = 0.0314; SjCRT: t (3) = 11.93, P = 0.0070; CD86: LPS t (3) = 14.73, P = 0.0046; SjCRT: t (3) = 3.486, P = 0.0252. Supernatants were collected for ELISA analysis of IL-4, IL-10, TNF-α and IFN-γ secretion. PBS was the negative control, and LPS was the positive control. Data illustrating representative experiments derived from triplicate experiments (a1-a2) and mean fluorescence intensities (b) are shown. The production of cytokines is shown (c1-c4) TNF-α: LPS t (5) = 29.13, P = 0.0012, SjCRT: t (5) = 65.63, P = 0.0002; IFN-γ: LPS t (5) = 25.48, P < 0.0001, SjCRT t (5) = 10.70, P = 0.0017; IL-4: LPS t (5) = 23.78, P < 0.0001, SjCRT t (5) = 13.45, P = 0.0001; IL-10: LPS t (5) = 3.472 P = 0.0403, SjCRT t (5) = 3.294, P = 0.0460. Statistically significant differences compared with the negative control group are shown as *P < 0.05 or **P < 0.01

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