Fig. 6

Effect of rSjCRT on lymphocytes in mice. Splenocytes from immunized mice were re-stimulated with rSjCRT or PBS at 37 °C under 5% CO₂ for 72 h. Splenocyte proliferation was assayed by MTT (a) SjCRT: t ₍₃₎ = 20.44, P = 0.0003; ConA: t ₍₃₎ = 6.888, P = 0.0063. The supernatants were collected, and the concentrations of IFN-γ, IL-4, IL-10 and TNF-α were measured by ELISA (b) IFN-γ: t ₍₃₎ = 29.63, P < 0.0001; IL-4: t ₍₃₎ = 6.295, P = 0.0081; IL-10: t ₍₃₎ = 1.510, P = 0.2283; TNF-α: t ₍₃₎ = 41.39, P < 0.0001. The re-stimulated cells were analyzed as the ratio of CD4⁺/CD8⁺ T cells by FACS. Data illustrating representative experiments derived from quadruplicate experiments are shown as a graph (c1-c2) and the ratio of CD4⁺/CD8⁺ (d) (t ₍₄₎ = 3.422, P = 0.0267). The production of intracellular cytokines IL-4 and IFN-γ by CD4+ T cells was measured by FACS (e) IFN-γ: t ₍₃₎ = 15.67, P = 0.0006; IL-4: t ₍₃₎ = 2.031, P = 0.1352. Statistically significant differences compared with the PBS group are shown as *P < 0.05 or **P < 0.01