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Table 1 The numbers of snails sampled from the Gombe ecosystem and analysed for trematode infections by cercarial shedding and PCR. Infection prevalence is expressed as percent of snails screened; also indicated is the prevalence of another trematode (family Derogenidae), with a 1000 bp PCR product

From: Assessing S. mansoni prevalence in Biomphalaria snails in the Gombe ecosystem of western Tanzania: the importance of DNA sequence data for clarifying species identification

Villagea No. of snails sampled Prevalence S. mansoni (shedding)b No. of snails screened (PCR)c Prevalence (500 bp band)c Prevalence (1000 bp band)
Bugamba 23 0 23 34.8 26.1
Gombe 46 0 32 0.08 0
Kigoma 30 26.7 30 55.6 0
Kiziba 28 24.1 28 54.2 0
Mwamgongo 108 11.4 106 58.1 22.6
Total/average 235 12.4 219 46.9 13.8
  1. a Mtanga village has been excluded from this table, as no snails were found
  2. b All collected snails were tested for cercarial shedding and all shed cercaria confirmed to be S. mansoni based on morphology
  3. c Species confirmation through sequencing was made for 8 individuals from Mwamgongo village, one individual each from Kiziba and Bugamba and two individuals from Gombe National Park. For Kigoma municipality, all three products sequenced were from an unknown trematode rather than S. mansoni
  4. Percentage prevalence based on PCR excludes individuals that did not show clear amplification of the snail band
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Parasites & Vectors

ISSN: 1756-3305

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