Table 1 PCR primes for genotyping ROP16, GRA3 and GRA15
From: Genotyping of polymorphic effectors of Toxoplasma gondii isolates from China
Marker | External primers for multiplex PCR | Internal primers for nested PCR | Restriction digestion |
|---|---|---|---|
ROP16 | ROP16-Fext: TCGTCCCGAATGCTGATGCCACGTC | ROP16-Fint: AAGCAACCGTGGTACGTCGAGGTTC | No restriction enzyme needed. Sequencing using double deoxidizing terminal cessation method |
ROP16-Rext: ATGCCCAAAGCCGTGGACATCGATC | ROP16-Rint: TCCATGCGCGAATCCAAGTTCGTG | ||
GRA15 | GRA15-Fext2: GCGTACATGGTTATGCGACG | GRA15-Fint2: GGTCATTGTCTGCAGACTGAT | No restriction enzyme needed. Sequencing using double deoxidizing terminal cessation method |
GRA15-Rext2: CATTCCAGTCCCTAAGTTCCCT | GRA15-Rint1: CCCTTATCGGTTTTTGGTCA | ||
GRA3 | GRA3-Fext: CCTTATTTAATGTTAGATCATCCCG | GRA3-Fint: AGGTACGCGTCGAGTAACCAGT | No restriction enzyme needed. Sequencing using double deoxidizing terminal cessation method |
GRA3-Rext: ACACCCGGTAGCAAGCGTTCA | GRA3-Rint: CCCGAGAGAGACTGGCACGA |