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Fig. 6 | Parasites & Vectors

Fig. 6

From: A mechanistic study of Toxoplasma gondii ROP18 inhibiting differentiation of C17.2 neural stem cells

Fig. 6

Activity of the Wnt/β-catenin signaling pathway in C17.2 NSCs detected by TOP flash/FOP flash dual-luciferase report system. C17.2 NSCs were transfected with TOP flash/ FOP flash plasmid and pRL-SV40 vector, followed by infection with or without Ad-ROP18 at MOI of 70, and then were cultured in DMEM: F12 supplemented with 2% N2 for 3 or 5 days. The activities of firefly luciferase and renilla luciferase were measured by a luminometer, and the activity of the Wnt/β-catenin signaling pathway was recorded as TOP/FOP. All these data represent the mean ± SD on different assays (n = 3), *P < 0.05. Abbreviations: Non, non-infection group, C17.2 NSCs without Ad-ROP18/Ad infection; Ad, C17.2 NSCs infected with adenovirus empty vector; Ad-ROP18, C17.2 NSCs infected with Ad-ROP18 recombinant adenovirus

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