Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

Table 1 List of primers used in the SYBR green-based qRT-PCR analysis

Gene target		Primer sequence (5′–3′)	Product length (bp)	Reference
GAPDH	F	CCTGCACCACCAACTGCTTG	222	[62]
GAPDH	R	TTGAGCTCAGGGATGACCTTG	222	[62]
IFN-γ	F	GTCTCCTTCTACTTCAAACT	253	[63]
IFN-γ	R	ATTCTGACTTCTCTTCCGCT	253	[63]
TGF-β	F	CGTGCTAATGGTGGAATAC	208	Present study
TGF-β	R	GCCAGGAATTGTTGCTATA	208	Present study
IL-1β	F	CTAGCCCATGTGTGCTGAAG	59	[62]
IL-1β	R	CCTTTACTTGGCTCTTCACC	59	[62]
IL-4	F	CAGCATGGAGCTGCCT	177	[64]
IL-4	R	ACAGAACAGGTCTTGCTTGC	177	[64]
IL-6	F	CTGCAATGAGAAAGGAGATA	191	[63]
IL-6	R	GGTAGTCCAGGTATATCTGA	191	[63]
IL-10	F	CTGTGCCTCTCCCCTAGAGT	236	[62]
IL-10	R	GCAGCTAGCTCCACAAGGAA	236	[62]
IL-12B	F	CAGGGACATCATCAAACCAG	213	[63]
IL-12B	R	CTTGTGGCATGT GACTTTGG	213	[63]
IL-13	F	AGAACCAGAAGGTGCCGCT	50	[65]
IL-13	R	GGTTGAGGCTCCACACCATG	50	[65]
IL-17A	F	CTACAGTGAACTGGAAGGAAC	554	Present study
IL-17A	R	AAAAGGGGCTGGGTCT	554	Present study
T-bet	F	CCTGGACCCAACTGTCAACT	171	[66]
T-bet	R	GAAACTCGGCCTCATAGCTG	171	[66]
GATA-3	F	GATCAAGCCCAAGCGAAGG	124	Present study
GATA-3	R	CCGCAGGCATTGCAGACA	124	Present study
Foxp3	F	GACAGCACCCTTTCGACTGT	191	[66]
Foxp3	R	CTCCAGAGATTGCACCACCT	191	[66]
ROR-γτ	F	CTACAGTGAACTGGAAGGAAC	554	Present study
ROR-γτ	R	AAAAGGGGCTGGGTCT	554	Present study

ISSN: 1756-3305