Table 1 List of primers used in the SYBR green-based qRT-PCR analysis
Gene target | Primer sequence (5′–3′) | Product length (bp) | Reference | |
|---|---|---|---|---|
GAPDH | F | CCTGCACCACCAACTGCTTG | 222 | [62] |
R | TTGAGCTCAGGGATGACCTTG | |||
IFN-γ | F | GTCTCCTTCTACTTCAAACT | 253 | [63] |
R | ATTCTGACTTCTCTTCCGCT | |||
TGF-β | F | CGTGCTAATGGTGGAATAC | 208 | Present study |
R | GCCAGGAATTGTTGCTATA | |||
IL-1β | F | CTAGCCCATGTGTGCTGAAG | 59 | [62] |
R | CCTTTACTTGGCTCTTCACC | |||
IL-4 | F | CAGCATGGAGCTGCCT | 177 | [64] |
R | ACAGAACAGGTCTTGCTTGC | |||
IL-6 | F | CTGCAATGAGAAAGGAGATA | 191 | [63] |
R | GGTAGTCCAGGTATATCTGA | |||
IL-10 | F | CTGTGCCTCTCCCCTAGAGT | 236 | [62] |
R | GCAGCTAGCTCCACAAGGAA | |||
IL-12B | F | CAGGGACATCATCAAACCAG | 213 | [63] |
R | CTTGTGGCATGT GACTTTGG | |||
IL-13 | F | AGAACCAGAAGGTGCCGCT | 50 | [65] |
R | GGTTGAGGCTCCACACCATG | |||
IL-17A | F | CTACAGTGAACTGGAAGGAAC | 554 | Present study |
R | AAAAGGGGCTGGGTCT | |||
T-bet | F | CCTGGACCCAACTGTCAACT | 171 | [66] |
R | GAAACTCGGCCTCATAGCTG | |||
GATA-3 | F | GATCAAGCCCAAGCGAAGG | 124 | Present study |
R | CCGCAGGCATTGCAGACA | |||
Foxp3 | F | GACAGCACCCTTTCGACTGT | 191 | [66] |
R | CTCCAGAGATTGCACCACCT | |||
ROR-γτ | F | CTACAGTGAACTGGAAGGAAC | 554 | Present study |
R | AAAAGGGGCTGGGTCT | |||