Skip to main content

Table 4 Relative gene expression in the liver of buffaloes experimentally infected with F. gigantica compared to the control group. The relative quantities were obtained according to the comparative Cq method (2–ΔΔCq). P values indicate statistical significance (P < 0.05)

From: Dynamic expression of cytokine and transcription factor genes during experimental Fasciola gigantica infection in buffaloes

Gene Target

ANOVA

Multiple comparisons

3 dpi

10 dpi

28 dpi

42 dpi

70 dpi

98 dpi

F (DFn, DFd)

P

RQ

P

RQ

P

RQ

P

RQ

P

RQ

P

RQ

P

IFN-γ

4.693(6, 21)

0.0035

2.08

0.4326

5.51

0.1282

2.06

0.7358

3.21

0.4590

9.27

0.0079

13.91

0.0003

TGF-β

4.738(6, 19)

0.0041

13.93

0.0003

3.07

0.4884

1.67

0.8036

3.85

0.3408

1.63

0.8371

2.66

0.6062

IL-1β

11.05(6, 26)

< 0.0001

1.31

0.9767

1.27

0.9811

14.25

0.0007

0.26

0.8137

2.31

0.8025

28.45

< 0.0001

IL-4

2.877(6, 24)

0.0291

7.01

0.5847

19.4

0.4892

97.21

0.0028

27.9

0.3121

9.43

0.7749

67.14

0.0630

IL-6

7.636(6, 24)

0.0001

5.50

0.8529

2.81

0.9366

78.1

0.0002

1.94

0.9728

23.14

0.2805

94.56

0.0008

IL-10

2.514(6, 26)

0.0471

5.99

0.7003

9.92

0.3184

21.18

0.0694

2.3

0.9579

6.24

0.4902

32.67

0.0036

IL-12B

5.595(6, 26)

0.0008

5.92

0.4786

3.99

0.4746

13.68

0.0759

0.86

0.9825

5.37

0.5312

24.84

< 0.0001

IL-13

6.56(6, 20)

0.0006

2.09

0.8459

7.80

0.1522

11.87

0.0259

5.45

0.3499

10.8

0.0426

27.33

< 0.0001

IL-17A

3.532(6, 21)

0.0142

0.33

0.4612

0.07

0.3694

5.95

0.0114

0.26

0.4327

0.28

0.4400

2.99

0.6354

T-bet

2.173(6, 28)

0.076

2.16

0.7923

0.25

0.7830

4.73

0.1018

3.93

0.4158

3.99

0.4606

11.38

0.0113

GATA-3

3.319(6, 28)

0.0135

0.84

0.8101

1.42

0.9579

5.18

0.0035

4.80

0.0889

1.34

0.9397

1.8

0.6952

Foxp3

1.455(6, 28)

0.2296

0.70

0.9054

1.07

0.9749

3.03

0.0424

3.97

0.3705

3.40

0.4458

3.05

0.1372

ROR-γτ

4.387(6, 28)

0.003

2.25

0.7567

4.57

0.3780

10.28

0.0002

3.24

0.5637

2.68

0.7076

2.55

0.7148

  1. Abbreviations: RQ, relative quantity (mean); F(DFn, DFd), degrees of freedom with numerator and degrees of freedom denominator inside the subscript parentheses