Fig. 1
From: Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9
Plasmid map of pL6cs-hDHFR-ck2α. The HA-TY1 tag coding sequences were placed in the middle of the left and right homologous arms by the bridging PCR method. This continuous DNA fragment was then inserted into the pL6cs-hDHFR vector linearized with AscI and AflII by using an in-fusion method. The plasmid map of pL6cs-hDHFR-ck2α was used as a map example. The plasmid maps offering donor DNAs for ck2β1 and stk are similar to this ck2α map, except homologous arms and sgRNA expressing cassettes are gene specific