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Fig. 3 | Parasites & Vectors

Fig. 3

From: Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9

Fig. 3

Genomic DNA PCR to confirm the tagging. Using genomic DNA as the template and specific primers of each gene (P33/P34 for ck2β1, P1/P2 for ck2α, P35/P36 for stk), the products obtained by PCR were separated on an agarose gel. The negative control was the PCR product obtained from 3D7 genomic DNA. The PCR product sizes of ctrl-1, ctrl-2 and ctrl-3, were 1175 bp, 1437 bp, and 1390 bp, respectively. After tagging, the PCR product sizes of transgenic ck2β1-HA, ck2α-HA-TY1 and stk-HA-TY1 were 1280 bp, 1650 bp, and 1603 bp, respectively

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