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Fig. 4 | Parasites & Vectors

Fig. 4

From: Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9

Fig. 4

Western blot to confirm the tagging. The tags added to CK2α, CK2β1 and STK were HA-TY1, HA, HA-TY1, respectively. Therefore, mouse anti-TY1, rabbit anti-HA and mouse anti-TY1 were 1st antibodies used for the Western Blot of CK2α-HA-TY1, CK2β1-HA, STK-HA-TY1, to confirm the successful tagging. The same lysate samples were also separated on SDS-PAGE and blotted with mouse anti-Actin as the loading control. HRP-goat anti-mouse and HRP-goat anti-rabbit were the second antibodies

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