Fig. 5
From: Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9
Subcellular localization of STK protein by immunofluorescence assay. Parasites at ring, trophozoite and schizont stages were collected and incubated with mouse anti-TY1 antibody and then 2nd antibody. The red fluorescence represents STK-HA-TY1; the blue fluorescence is DAPI, representing the parasite nucleus. In the images at top line, the picture highlighted by square with dashed line is IFA of a nucleus taken under other view