Fig. 2From: Molecular screening of tsetse flies and cattle reveal different Trypanosoma species including T. grayi and T. theileri in northern CameroonITS1 amplicons of Trypanosoma species. a Amplicon sizes expected for amplification with generic primers ITS1-InF and ITS1-InR were calculated from available database sequences and crosschecked with sequences from screened samples. b PCR was performed with representative samples containing DNA of the indicated origin and generic (a) or specific primers (Table 1). Lane M: Marker GeneRuler 50Â bp Ladder (Thermo Scientific); Lane 1: NI100 (generic primers); Lane 2: Bodonid (generic primers); Lane 3: T. vivax (generic primers); Lane 4: T. grayi (generic primers); Lane 5: T. theileri (generic primers); Lane 6: T. brucei ssp. (generic primers); Lane 7: T. grayi (specific primers); Lane 8: T. congolense (specific primers); Lane 9: T. congolense forest (specific primers); Lane 10: T. congolense forest and T. congolense kilifi (specific primers); Lane C: control without DNA templateBack to article page