Fig. 4
From: Molecular and biochemical characterization of calmodulin from Echinococcus granulosus
Immunohistochemical localization of E. granulosus calmodulin (EgCaM) in sections of E. granulosus. Anti-recombinant EgCaM (rEgCaM) rabbit IgG was used as the primary antibody and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG was used as the secondary antibody to detect the native protein in protoscoleces, the germinal layer, and adult worms. The nuclear DNA was stained with 2-(4-amidinophenyl)-1H–indole-6-carboxamidine (DAPI). Pre-immune rabbit sera was used as negative control. Images of protoscoleces are magnified at ×400; germinal layer and adult worm are magnified at ×200. Abbreviations: LL, laminated layer; GL, germinal layer; Teg, tegument; PR, parenchymal region