Fig. 5

Relative expression levels of recombinant E. granulosus calmodulin mRNA (rEgCaM) in PSCs treated with H₂O₂ for different times. The mRNA expression of rEgCaM in PSCs treated with 5 mM H₂O₂ for 0, 1, 2, 3, 4, 5 and 6 h were assessed by qPCR analysis and normalized to the levels of the β-actin gene. Data are presented as the mean ± SD of triplicate experiments. Statistically significant differences between the 0 h group (as the control) and the other groups were determined using Student’s t-test (2 h: t ₍₇₎ = 3.832, P = 0.005; 6 h: t ₍₇₎ = -2.792, P = 0.000) (*P < 0.05*)