Table 2 Primers used for PCR and RT-PCR reactions in this study
From: Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression
Accession no. | Gene | Direction | Sequence | Amplicon size (bp) | Source |
|---|---|---|---|---|---|
Genotyping primers | |||||
JDP | ASA.1 | Forward | 5′-GGCCCAGATCGTTTACCGTGAA-3′ | 423–551 | [11] |
Reverse | 5′-TCTCACAAGCTGCTAGGGAGTCA-3′ | ||||
Acanthamoeba virulent marker primers | |||||
U29609 | ARP2 | Forward | 5′-GCTGTCTTGACCCTCTACGC-3′ | 101 | Present study |
Reverse | 5′-AGCGAGAAGCCCTCGTACAC-3′ | ||||
AY604040 | MBP | Forward | 5′-AGGGCGAGACCTACGATAGC-3′ | 165 | Present study |
Reverse | 5′-CCTCGTAGACGAAGGTGAGG-3′ | ||||
AY351649 | AhLBP | Forward | 5′-CCAACACCGACTCTCCTCTC-3′ | 183 | Present study |
Reverse | 5′-CTCCTCAGGGTCACGGTAGA-3′ | ||||