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Fig. 2 | Parasites & Vectors

Fig. 2

From: A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA

Fig. 2

Using the LAMP assay for detecting N. caninum plasmid DNA containing the Nc-5 region on a SYBRsafe stained agarose gel. a The effects of reaction time. Lanes 1 and 2: 60 min; Lanes 3 and 4: 50 min; Lanes 5 and 6: 40 min. Lanes 2, 4 and 6 represent negative controls for each reaction time. b Optimizing LAMP assay conditions. Lanes 1 and 2: 30 min; Lanes 3 and 4: 20 min. Lanes 2 and 4 are the negative control for each reaction time. c Restriction analysis of N. caninum LAMP products amplified from plasmid DNA containing the Nc-5 region. The digestion products were run on a 3% agarose gel. Lane 1: N. caninum LAMP product; Lane 2: MspI digestion of N. caninum product (132–218 bp bands, according to predicted size). Lane MM in a, b and c is the HyperLadder II (Bioline) DNA molecular marker

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