Skip to main content

Table 1 A list of primers used for the LAMP method

From: A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA

Primer name

Type

Length

Localizationa

Sequence (5′–3′)

F3

Forward outer

20

73–92

TGAACCGAGGGAGTTGGTAG

B3

Reverse outer

18

269–286

ACGTCTTCTGCCCCTTCC

FIPb

Forward inner

40

F1C, 140–160

ACCCTGACGCAGGCTGATTTC

 

(FIP = F1C + F2)

 

F2, 97–115

GAGAGGTGGGATACGTGGT

BIPb

Reverse inner

40

B1C, 200–221

TTCTGTGTTGAGGCAACACCGG

 

(BIP = B1C + B2)

 

B2, 248–265

GTCCGCTTGCTCCCTATG

FLP

Forward Loop

24

116–139

AACGTGACGAATGACTAACCACAA

BLP

Reverse Loop

21

223–243

GGCACTGATGACGGGGGAGAT

  1. aLocalization of the primers is based on the nucleotide sequence of N. caninum Nc-5 unique repetitive region (GenBank: AY459289.1)
  2. bEach inner primer of LAMP contains two connected primers