Fig. 3
From: Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity
Analysis of RNA-binding capacity by pull down assays. a Analysis of rLbSCD6. b Analysis of rLbRBP42. Two hundred nanograms of digoxigenin labeled HSP70-II 3′ UTR were incubated with beads containing different amounts of protein. After washing out of the unbound RNA, eluted complexes were deposited on the membrane and the bound RNA was monitored by an antidigoxigenin antibody. RPA1 protein (6.25 pM) was used as a positive control (PC) [23], and Lbα-tubulin as a negative control (NC)