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Fig. 1 | Parasites & Vectors

Fig. 1

From: Culex pipiens pallens cuticular protein CPLCG5 participates in pyrethroid resistance by forming a rigid matrix

Fig. 1

Identification of the CPLCG protein CPLCG5 in leg extracts from Culex pipiens pallens. a Analysis of cuticle protein leg extracts from 3-day-old adult mosquitoes (n = 40) by gradient gel electrophoresis (4−20%). The 12.7 kDa band was excised, digested with trypsin, and analysed by LC-MS/MS. b Nucleotide and deduced amino acid sequences of CPLCG5. The protein band was excised from the gel, digested with trypsin, and resulting peptides were analysed by LC-MS/MS. Proteins with two matched peptides are highlighted in grey, and include N103-K119 and N121-K129. N1-K18 is a predicted signal peptide. c, d, Western blotting analysis of CPLCG5 revealing ~2.2-fold higher protein levels in the DR strain than the DS strain (t-test, t(4) = -4.917, P = 0.008; **P < 0.01)

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