Fig. 4
siRNA-mediated downregulation of CPLCG5. siCPLCG5 (500 ng per insect) was injected into the thorax of 12 h female adult DR mosquitoes, and animals were recovered for 3 days. Expression of CPLCG5 was analysed by real-time PCR (a) and western blotting (b). a cDNAs were prepared from total RNA isolated from 3-day-old adults. The results indicate a significant difference in CPLCG5 transcript levels between control and experimental groups (t-test, t(4) = 4.244, P = 0.013; *P < 0.05). Data are means ± SE. b Proteins were extracted from the legs of 3-day-old adults (n = 20) from each group. CPLCG5 was significantly and specifically downregulated at the protein level following siRNA injection. c Insecticide resistance level after CPLCG5 knockdown analysed by CDC bottle assays. The results indicate that the knockdown rate of the siCPLCG5 group is higher than that of the siNC control group (Chi-square test, Additional file 5; *P < 0.05, **P < 0.01)