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Fig. 3 | Parasites & Vectors

Fig. 3

From: Immune-related redox metabolism of embryonic cells of the tick Rhipicephalus microplus (BME26) in response to infection with Anaplasma marginale

Fig. 3

Effect of RNAi-mediated silencing of Rmoxr1 in the control of infection of BME26 cells by Anaplasma marginale. BME26 cells were incubated with Rmds oxr1 or with Pfdsmsp1 as the control for 24 h and then infected with A. marginale for 72 h. The gene expression of BME26 cells was determined using RT-qPCR, and the number of A. marginale was determined using qPCR. a Relative quantitation of the mRNA levels of Rmoxr1 in BME26 cells after incubation with dsRNAs compared with cells incubated with msp1 dsRNA (control). b A. marginale cells per flask at 72 h post-incubation in dsRNA-treated BME26 cells. The dots represent the relative mRNA levels or the number of A. marginale in individual flasks (eight biological replicates), the horizontal line indicates the median relative mRNA level or the number of A. marginale cells, and bars represent the standard deviation. The percentage of gene silencing is indicated in A. The asterisk indicates significant differences by Mann-Whitney test (a U (14) = 0, Z = -3.36, P = 0.0002 and b U (14) = 6, Z = -2.73, P = 0.0047)

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