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Fig. 2 | Parasites & Vectors

Fig. 2

From: Selection strategy of phage-displayed immunogens based on an in vitro evaluation of the Th1 response of PBMCs and their potential use as a vaccine against Leishmania infantum infection

Fig. 2

Immunoblotting assays. A molecular weight marker (Lane a), 20 μg L. infantum SLA (Lane b) and 10 μg rLiHyp (Lanes c, d) were electrophoresed on a 20% SDS-PAGE and blotted onto nitrocellulose membranes. Blots were incubated with pools of sera from B1 phage-vaccinated mice (Lanes b and c) or from naive mice (lane d), and were revealed by adding chloronaphtol, diaminobenzidine, and H2O2. A scan from the blots is shown here. The black arrow indicates the rLiHyp protein (~28.0 kDa)

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Parasites & Vectors

ISSN: 1756-3305

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