Anaplasma phagocytophilum in the highly endangered Père David’s deer Elaphurus davidianus

Parasites & Vectors

Table 1 Oligonucleotide sequences of the primers used in this study

Pathogen	Target gene	Primer		Amplicon size (bp)	References
Pathogen	Target gene	Primer Name	Oligonucleotide sequence (5′-3′)	Amplicon size (bp)	References
A. centrale	16S rRNA	AC1f	CTGCTTTTAATACTGCAGGACTA	426	Kawahara et al., [6]
A. centrale	16S rRNA	AC1r	ATGCAGCACCTGTGTGAGGT	426	Kawahara et al., [6]
A. bovis	16S rRNA	AB1f	CTCGTAGCTTGCTATGAGAAC	551	Kawahara et al., [6]
A. bovis	16S rRNA	AB1r	TCTCCCGGACTCCAGTCTG	551	Kawahara et al., [6]
A. phagocytophilum	16S rRNA	SSAP2f	GCTGAATGTGGGGATAATTTAT	641	Kawahara et al., [6]
A. phagocytophilum	16S rRNA	SSAP2r	ATGGCTGCTTCCTTTCGGTTA	641	Kawahara et al., [6]
A. marginale	msp4	Amargmsp4 F	CTGAAGGGGGAGTAATGGG	344	Torina et al., [8]
A. marginale	msp4	Amargmsp4 R	GGTAATAGCTGCCAGAGATTCC	344	Torina et al., [8]
A. ovis	msp4	Aovismsp4 F	TGAAGGGAGCGGGGTCATGGG	347	Torina et al., [8]
A. ovis	msp4	Aovismsp4 R	GAGTAATTGCAGCCAGGGACTCT	347	Torina et al., [8]
A. platys	16S rRNA	Platys-F	AAGTCGAACGGATTTTTGTC	504	Inokuma et al., [7]
A. platys	16S rRNA	Platys-R	CTTTAACTTACCGAACC	504	Inokuma et al., [7]

ISSN: 1756-3305