Table 1 Antibodies and reagents used for immunohistochemistry
Primary antibody | Antibody type | Dilution | Supplier | Epitope demasking method | Link antibody (dilution) | Buffer |
|---|---|---|---|---|---|---|
CD3 | Rabbit vs Human CD3 (polyclonal) | 1/500 | Dako (A0452) (Glostrup, Denmark) | Proteinase K (Dako) | Goat vs Rabbit (1/1000) | TBS |
CD45R (B220) | Rat vs Mouse CD45R (B220) (monoclonal) | 1/100 | Invitrogen (RA3-6B2) (Thermo Fisher, Darmstadt, Germany) | pHÂ 6 citrate-buffer (Dako) | Goat vs Rat (1/200) | TBS |
Ly-6G (Gr1) | Rat vs Mouse Ly-6G (Gr1) (monoclonal) | 1/25 | Invitrogen (RB6-8C5) | pHÂ 6 citrate-buffer (Dako) | Goat vs Rat (1/200) | TBS |
F4/80 | Rat vs Mouse F4/80 (monoclonal) | 1/100 | BioRad (MCA 4976) (Watford, Hertfordshire, UK) | None (ZSF samples) | Goat vs Rat (1/200) | TBS |
iNOS | Rabbit vs Mouse iNOS (polyclonal) | 1/500 | Merck Millipore (ABN26) (Watford, Hertfordshire, UK) | None (ZSF samples) | Goat vs Rabbit (1/1000) | TBS |
Anti-Leish | Rabbit vs Leishmania spp. | 1/50 | ISCIII (Madrid, Spain) | None | Goat vs Rabbit (1/1000) | TBS |