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Table 5 Genetic diversity observed at each locus. Amplicon lengths with external or internal primers were obtained with Primer-BLAST (NCBI), apart from the PCR products with recG external primers, and polA external and internal primers, which were estimated on agarose gels

From: Co-circulation of different A. phagocytophilum variants within cattle herds and possible reservoir role for cattle

  typA ctrA msp4 pleD recG polA
No. of complete sequences 40 39 36 36 35 37
No. of sequences without ambiguities 31 39 28 33 33 31
No. of sequences with ambiguities (%) 9 (22.5) 0 (0) 8 (22.2) 3 (8.3) 2 (5.7) 6 (16.2)
Amplicon length with external primers (bp) 1455 1453 558 1102 515 ~490
Amplicon length with internal primers (bp) 550 574 471 na ~480 ~460
Alignment length (bp) 385 317 337 597 309 331
No. of polymorphic sites 5 6 11 20 6 7
Proportion of polymorphic sites 1.30 1.89 3.26 3.35 1.94 2.11
No. of alleles at each locus 7 6 7 3 8 8
  1. Abbreviation: na not applicable