Fig. 4

Quantification of distinct forms of T. cruzi during metacyclogenesis. The percentage of cells are counted at different time points during differentiation. Note that as metacyclogenesis proceeds, the number of epimastigotes and adhered parasites decreases to generate free metacyclic trypomastigotes in the supernatant. The largest number of intermediate forms are observed after 72 h of differentiation. Data were obtained from three independent experiments