Fig. 6

Three-dimensional reconstruction of T. cruzi forms during metacyclogenesis. a In epimastigotes, the disk-shaped kinetoplast and the flagellum can be seen in the anterior region of the parasite, and the nucleus appears rounded. b-e Repositioning of the flagellum-kinetoplast complex from the anterior to the posterior end of the parasite cell body. During kinetoplast relocation, it contacts the nucleus, which deforms and elongates. The globular kinetoplast can be observed during late stage of differentiation (f) and in trypomastigotes (g). The kinetoplast (green), nucleus (blue), flagellum (yellow), cell membrane (gray), and nucleolus (light blue) are shown. Scale-bars: a-g, 2.5 μm; a’-g’, 1 μm