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Fig. 4 | Parasites & Vectors

Fig. 4

From: Cysteine peptidases of Eudiplozoon nipponicum: a broad repertoire of structurally assorted cathepsins L in contrast to the scarcity of cathepsins B in an invasive species of haematophagous monogenean of common carp

Fig. 4

SDS-PAGE and active site labelling of purified pro-yrEnCL3 after auto-activation at various pH. The 38 kDa zymogen was autocatalytically processed to a 28 kDa form during incubation in 50 mM/100 mM CPB containing 2 mM DTT at 37 °C. After incubation at various pH values, the protein was run in 12% polyacrylamide gels and stained with Coomassie Brilliant Blue (pH 4, pH 5 and pH 6). Lane 1: 15 min incubation; Lane 2: 30 min; Lane 3: 60 min; Lane 4120 min. DCG-04, a 12% gel showing active site labelling of the processed enzyme with fluorescent affinity probe BODIPY green DCG-04. Lane 1: labelling by the affinity probe after 30 min incubation at pH 5; 1*, binding of the affinity probe was blocked by E-64 inhibitor. The 38 kDa zymogen and the 28 kDa processed enzyme are indicated by arrows

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