Fig. 6From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feedingExpression profiles of GSTs in different tick stages (a) and organs (b) during blood-feeding. Proteins were prepared from different stages and tissues after dissection. Antiserum against tubulin was used as a control for Western blotting. Western blotting results are shown as representative data of three separate experiments showing the same trend. Since no band can be seen using the tubulin antisera on hemocytes, the protein concentration was determined using Micro BCA and maintained at 390 ng before loading for Western blottingBack to article page