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Fig. 7 | Parasites & Vectors

Fig. 7

From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding

Fig. 7

Localization of GSTs in tissues of partially fed adult ticks. Immunofluorescent antibody test (IFAT) was used to determine the localization of the GSTs in the different tissues of ticks. Antiserum against HlGST or HlGST2 was used for the primary antibody, anti-mouse IgG conjugated with Alexa 488 was used for the secondary antibody, and nuclei were visualized using DAPI. Normal mouse serum was used for a control. The tissues were visualized using confocal microscope. Abbreviations: Salivary glands (A, acinus; D, salivary ducts); Midgut (E, enterocytes; L, lumen; Fat bodies (T, tracheal complex; Fb, fat body cells); Ovary (O, oocyte; Pd, pedicel; Ow, ovarian wall). Arrows show positive GST fluorescence. Scale-bars: 20 μm

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