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Fig. 8 | Parasites & Vectors

Fig. 8

From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding

Fig. 8

Examination of HlGST (a) and HlGST2 (b) in selected tissues during blood-feeding. The salivary glands, midgut, and ovary were observed during blood-feeding of adult ticks by indirect immunofluorescent antibody test (IFAT) using a confocal laser scanning microscope. Antiserum against HlGST or HlGST2 was used for the primary antibody, anti-mouse IgG conjugated with Alexa 488 was used for the secondary antibody, and nuclei were visualized using DAPI. Normal mouse serum was used for a control. Abbreviations: Salivary glands (A, acinus; D, salivary ducts); Midgut (E, enterocytes; L, lumen); Ovary (O, oocyte; Pd, pedicel; Ow, ovarian wall). Arrows show the positive fluorescence of GST. Scale-bars: 20 μm

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Parasites & Vectors

ISSN: 1756-3305

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