Table 1 Gene-specific primers used in this study. Underlined letters indicate enzyme recognition sites. RNA polymerase promoter sequence is indicated in italic
Primer | Sequence (5′ → 3′) |
|---|---|
HlGST real-time forward | CTTCTTGGATCTTGGCGGGT |
HlGST real-time reverse | CGATGTCCCAGTAGCCGAG |
HlGST BamHI forward | CGGGATCCATGGCTCCTATTCTCGGCT |
HlGST EcoRI reverse | CGGAATTCTCAGCAGTCGTCAGCGGGCG |
HlGST RT forward | ACGTGAAGCTCACCCAGAGCAT |
HlGST RT reverse | AAGCTAGCCATGTCGCCGTTGA |
HlGST RNAi forward | GCCTGGCTCAAGGAGAAACACA |
HlGST RNAi reverse | ACAAAGGCCTTCAGGTTGGGGA |
HlGST T7 forward | TAATACGACTCACTATAGGGCCTGGCTCAAGGAGAAACACA |
HlGST T7 reverse | TAATACGACTCACTATAGGACAAAGGCCTTCAGGTTGGGGA |
HlGST2 real-time forward | CCCTTCCGGGAATGAAGGAG |
HlGST2 real-time reverse | GATCGCTCAGCAGTCGTCAG |
HlGST2 BamHI forward | CGGGATCCATGGCCCCTGTGCTGGGATA |
HlGST2 EcoRI reverse | CGGAATTCTCAGCAGTCGTCAGCGGGCG |
HlGST2 RT forward | ACGTCAAGCTGACGCAGAGCAT |
HlGST2 RT reverse | ATGGGCCAAGCCTTGAAGCGAT |
HlGST2 RNAi forward | AGGATAAAAGGTACGGCTTCGGCA |
HlGST2 RNAi reverse | TTTCACGATCTGGAGAGCCTCGTA |
HlGST2 T7 forward | TAATACGACTCACTATAGGAGGATAAAAGGTACGGCTTCGGCA |
HlGST2 T7 reverse | TAATACGACTCACTATAGGTTTCACGATCTGGAGAGCCTCGTA |
P0 real-time forward | CTCCATTGTCAACGGTCTCA |
P0 real-time reverse | TCAGCCTCCTTGAAGGTGAT |
L23 real-time forward | CACACTCGTGTTCATCGTCC |
L23 real-time reverse | ATGAGTGTGTTCACGTTGGC |
Actin real-time forward | ATCCTGCGTCTCGACTTGG |
Actin real-time reverse | GCCGTGGTGGTGAAAGAGTAG |
Actin RT forward | CCAACAGGGAGAAGATGACG |
Actin RT reverse | ACAGGTCCTTACGGATGTCC |
Tubulin real-time forward | TTCAGGGGCCGTATGAGTAT |
Tubulin real-time reverse | TGTTGCAGACATCTTGAGGC |
EGFP T7 forward | TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTT |
EGFP T7 reverse | TAATACGACTCACTATAGGTGCTCAGGTAGTGGTTGTCG |