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Table 3 Recombinant GST kinetic constants. Values are presented as mean ± SD. Each experiment was performed at least three times and each assay was run in triplicate

From: Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood-feeding

Enzyme

CDNB kinetic constanta

GSH kinetic constantb

rHlGSTc

 V max (units/mg protein)

11.70 ± 1.92

10.40 ± 1.77

 K m (mM)

0.82 ± 0.14

0.64 ± 0.32

rHlGST2d

 V max (units/mg protein)

14.72 ± 0.56

11.01 ± 0.21

 K m (mM)

0.61 ± 0.20

0.53 ± 0.19

  1. aCDNB, the common substrate, is used in increasing concentration from 0.125–2 mM to determine the kinetic constants
  2. bGSH is used in increasing concentration for 0.5–5 mM to determine the kinetic constants
  3. cRecombinant HlGST
  4. dRecombinant HlGST2
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Parasites & Vectors

ISSN: 1756-3305

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