Fig. 3

Serum samples were tested for the presence of heartworm antigen monthly pre- and post-heat-treatment. Heat-treated samples were placed at 103 °C for 10 min and centrifuged at 1818× g for 20 min. The absorption values using heat-treatment were significantly lower (P < 0.05) from animals in the treated group as compared to the non-treated animals on study days 112 (t₍₁₇₎ = 2.56, P = 0.0205), 140 (t₍₂₂₎ = 2.69, P = 0.0133), 168 (t₍₁₈₎ = 2.12, P = 0.0479), 196 (t₍₂₃₎ = 3.63, P = 0.0014), 224 (t₍₂₀₎ = 3.26, P = 0.0039), 279 (t₍₄₎ = 6.76, P = 0.0031), 280 (t₍₄₎ = 5.16, P = 0.0074) and 281 (t₍₄₎ = 6.87, P = 0.0016) using a repeated measures analysis of variance having a significant treatment by time interaction (degrees of freedom were rounded for display purposes)