Fig. 5

Upregulated expression of the UNC93B1 gene by knock down of NONHSAT022487. a RT-qPCR analysis showing the transcriptional levels of NONHSAT022487 in various HFF cells as indicated. Cells were transfected with the shRNA-NONHSAT022487 lentiviral plasmid (sh-NONHSAT02248) or shRNA-control plasmid (sh-control). b As described in a, except that the transcriptional level of the UNC93B1 gene was detected. c Western blotting image showing the protein expression of UNC93B1 in various HFF cells as indicated. GAPDH was used for internal normalization. The ratio of UNC93B1/GAPDH indicated the relative expression changes in various treated HFF cells according to Western blotting. d As described in a, except that THP-1 cells were used. e As described in b, except that THP-1 cells were used. f As described in c, except that THP-1 cells were used. Data are the mean with standard deviations (SD) from three independent biological replicates. *P < 0.05, **P < 0.01. Abbreviations: NC, no significant changes