Fig. 6

Downregulated expression of the UNC93B1 gene by over-expression of NONHSAT022487. a RT-qPCR analysis showing the transcriptional level of NONHSAT022487 in various HFF cells as indicated. Cells were transfected with pcDNA3.1-NONHSAT022487 (pc-NONHSAT022487) or pcDNA control plasmid (pc-control). b As described in a, except that the transcriptional level of UNC93B1 was detected. c Western blotting image showing the protein expression of UNC93B1 in various HFF cells as indicated, the GAPDH was used for the internal normalization. The ratio of UNC93B1/GAPDH indicated the relative expression changes in various treated HFF cells according to the Western blot. d As described in a, except that THP-1 cells were used. e As described in b, except that THP-1 cells were used. f As described in c, except that THP-1 cells were used. Data are the mean with standard deviations (SD) from three independent biological replicates. *P < 0.05, **P < 0.01. Abbreviations: NC, no significant changes