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Fig. 3 | Parasites & Vectors

Fig. 3

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

Fig. 3

Graphical representation of the data obtained in the quantification of DNA-protein complexes shown in Fig. 2 (e and f). a, d Data corresponds to the natural logarithm of the radioactivity present in the DNA-protein complexes (ln S x ) as a function of the protein concentration x. Dots correspond to experimental data. The continuous line is the graph predicted by the second-degree polynomial function. b, e Mathematical relationships shown by the natural logarithm of the fraction (F) of DNA probe in GAAC-box-protein complexes and the rEhTRF1/GAAC-box molar ratios (b) and rEhTBP/GAAC-box molar ratio (e). Dots correspond to experimental data. Continuous line is the graph predicted by the second-degree polynomial function. c, f Mathematical relationships shown by the reciprocal of F (1/F) and the reciprocal of the total active uncomplexed protein P (1/P) for rEhTRF1 and rEhTBP, respectively. Dots correspond to experimental data. The continuous line is the graph predicted by the linear eq. 1/F = K D (1/P) + 1, where its slope corresponds to the K D value. The arrow indicates the complex with GAAC-like box

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