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Fig. 4 | Parasites & Vectors

Fig. 4

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

Fig. 4

Ehtbp is upregulated during nutrient deprivation. a Growth curve of trophozoites at 12, 18, 36, 72, 96 and 120 h. b, c Relative gene expression levels of Ehtbp (b) and Ehtrf1 (c) at different times of growth curve. Statistical significance (Student’s t-test) defined as *P < 0.05, **P < 0.001. d, e Images of trophozoites grown in TYI-S-33 medium for 12 h at 37 °C with serum (d) or without serum (e). Photomicrographs were obtained with a magnification of 200×. f RT-qPCR analysis of Ehtbp and Ehtrf1 genes. Statistical significance was determined using the Student’s t-test (****P < 0.0001). Scale-bar: 10 μm

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