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Table 1 Sequences of the oligonucleotides used in EMSA experiments

From: The Entamoeba histolytica TBP and TRF1 transcription factors are GAAC-box binding proteins, which display differential gene expression under different stress stimuli and during the interaction with mammalian cells

Oligonucleotide

Sequence (5′-3′)

TATTTAAA (1)

AATTCTCTATTTAAAGAGAATT

TAgTgAAA (2)

AATTCTCTAgTgAAAGAGAATT

TATTggAA (3)

AATTCTCTATTggAAGAGAATT

TATTaAAA (4)

AATTCTCTATTaAAAGAGAATT

TATgTAAA (5)

AATTCTCTATgTAAAGAGAATT

gAgTTAAA (6)

AATTCTCgAgTTAAAGAGAATT

TAcTcAAA (7)

AATTCTCTAcTcAAAGAGAATT

cAcTcAAA (8)

AATTCTCcAcTcAAAGAGAATT

cAcTTAAA (9)

AATTCTCcAcTTAAAGAGAATT

TATTTttt (10)

AATTCTCTATTTtttGAGAATT

GAAC-box (Wild type)

AAGACAATGAACTAGAATG

GAAC-boxa (Mutated)

AAGACCTACGATAAGAATG

GAAC-like boxb

AGGCGAACTACAAAAGAT

  1. aWild type and mutated GAAC-boxes are identical to those reported for hgl5 gene [33]
  2. bThis box was previously reported [17]
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ISSN: 1756-3305

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